Dr. Muhammad Anwar, Ph.D., is working as Associate Professor at Hainan University, Haikou, China. He has got his M.Sc. (Hons)degree fromArid Agriculture University Rawalpindi in 2008 and Ph.D. degree from Fujian Agriculture and Forestry University, Fuzhou 35002, China, in 2018. Hehas worked  as postdoctorate fellow Shenzhen University, Shenzhen (2019-2023). Hisresearch work is connectedwith molecular biology, gene cloning and functional characterization,genetic and metabolic engineering, heterologous protein overexpressionsystem, natural compounds and metabolites. He has presided various research funding projects.

• Molecular Biology
• Genetic and Metabolic Engineering
• Biosynthesis of Medicinal Compounds
• Bioinformatics Analysis, Transcriptomics, Metabolomics
• Genetic Transformation
• Identification, Cloning and Functional Characterization of R2R3-MYB Transcription Factors Involved in Flower Pigmentation, Colors Patterns and Plant Defense Mechanisms
• Agrobacterium-mediated Genetic Transformation in Chinese Narcissus Flower, Tobacco and Arabidopsis, Tissue Culture
• Transcriptional Regulation of the Flavonoid, Terpenoids Biosynthesis
• Biochemical Analysis of Secondary Metabolisms
• Gene Cloning, Over-expression and Transient Expression
• Molecular Breeding of Plants.
• Heterologous Protein Overexpression Expression System

• Muhammad Anwar, Weijun Yu, Hong Yao, Ping Zhou, Andrew C. Allan and Lihui Zeng.NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis. Int. J. Mol. Sci.2019, 20(21), 5456, November 2019. (6.2 IF)
• Anwar, M., Lou, S., Chen, L., Li, H., & Hu, Z. (2019). Recent advancement and strategy on bio-hydrogen production from photosynthetic microalgae. Bioresource technology, 121972. (11.88IF)
• Anwar, M.; Chen, L.; Xiao, Y.; Wu, J.; Li, H.; Wu, Q.; Zeng, L.; Hu, Z. Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid and Terpenoid Biosynthetic Pathway. Int. J. Mol. Sci. 2021, 22, x. https://doi.org/10.3390/x (6.2 IF).
• Altaf, Muhammad Mohsin, Sahrish Ashraf, Ahlam Khalofah, Muhammad Qaisar Naeem Khan, and Muhammad Anwar*. "Melatonin foliar application promoted anthocyanin accumulation boosted reactive oxygen scavenging by upregulating the key enzymes activities and genes expression of anthocyanin biosynthesis pathway in green and purple mustard under vanadium stress." Scientia Horticulturae 331 (2024): 113112 (4.3 IF). Corresponding author
• Shahid, Izzah, Salma Batool, Maleeha Hassan, Hammad Ismail, Samina Mehnaz, Farah Deeba, Muhammad Anwar*, Faisal Zulfiqar, Rashid Iqbal, and Hayssam M. Ali. "A Decade of Progress in Rhizoengineering to Exploit Plant Microbiome for Salinity Amelioration." Plant Stress (2023): 100325. (5.0 IF). Corresponding authors
• Arshad SF, Rehana R, Saleem MA, Usman M, Arshad HJ, Rizwana R, Shakeela S, Rukh AS, Khan IA, Hayssam MA, Anwar M*. Multi-epitopes vaccine design for surface glycoprotein against SARS-CoV-2 using immunoinformatic approach. Heliyon. 2024 Jan 30;10(2). (4.0 IF). Corresponding author
• Muhammad Anwar, Shuhua Duan, Mingjun Ma….Lihui Zeng.NataMYB4，a flower specific gene, regulates the flavonoid biosynthesis in Chinese narcissus. Scientia Horticulturae. 2023. xxxx(4.3 IF).
• Anwar, Muhammad, Jingkai Wang, Jiancheng Li, Muhammad Mohsin Altaf, and Zhangli Hu. "MYB Transcriptional Factors Affects Upstream and Downstream MEP Pathway and Triterpenoid Biosynthesis in Chlamydomonas reinhardtii." Processes 12, no. 3 (2024): 487. (3.5 IF)
• Anwar, M., Iqbal, M. Z., Abro, A. A., Memon, S., Bhutto, L. A., Memon, S. A., & Peng, Y. (2022). Inter-Specific Hybridization in Cotton (Gossypium hirsutum) for Crop Improvement. Agronomy, 12(12), 3158.. (3.7 IF)
• Anwar, M., Saleem, M.A., Dan, M., Malik, W., Ul-Allah, S., Ahmad, M.Q., Qayyum, A., Amjid, M.W., Zia, Z.U., Afzal, H. and Asif, M., 2021. Morphological, physiological and molecular assessment of cotton for drought tolerance under field conditions. Saudi Journal of Biological Sciences. (4.4 IF)
• Muhammad Anwar, Guiqing Wang , Jiacheng Wu , Saquib Waheed , Andrew C. Allan and Lihui Zeng . Ectopic overexpression of a novel R2R3-MYB, NtMYB2 from Chinese Narcissus represses anthocyanin biosynthesis in Tobacco.Molecules.23(4);781, March, 2018. (4.92IF)
• Anwar, M. *, Saleem, M.A., Wu, J., Tayyab, M. and Hu, Z., 2021. The Critical Role of Small RNAs in Regulating Plant Innate Immunity. Biomolecules 2021, 11, 184. (5.5 IF).
• Xiyan LOU 1, Muhammad Anwar*3, Yongqiang Wang, Huan Zhang, Jinping DING* 2. To investigate the effects of inorganic salts on vase life and postharvest qualities of the cut flower of Perpetual Carnation. Brazilian journal of biology.2019. https://doi.org/10.1590/1519-6984.221502 (1.65 IF) Corresponding author
• Guo, C., Mei, R., Anwar, M., Zhao, D., Lan, C., Jiang, Y., ... & Hu, Z. (2021). The Functionally Characterization of Putative Genes Involved in the Formation of Mannose in the Aplanospore Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta). Metabolites, 11(11), 725. (4.1IF).
• Fahad Raza, M., Anwar, M., Husain, A., Rizwan, M., Li, Z., Nie, H., ... & Su, S. (2022). Differential gene expression analysis following olfactory learning in honeybee (Apis mellifera L.). Plos one, 17(2), e0262441. (3.7 IF).
• Sajad, Shoukat, Shuhan Jiang, Muhammad Anwar, Qian Dai, Yuxia Luo, Muhammad A. Hassan, Charles Tetteh, and Jianghua Song. "Genome-Wide Study of Hsp90 Gene Family in Cabbage (Brassica oleracea var. capitata L.) and Their Imperative Roles in Response to Cold Stress." Frontiers in Plant Science 13 (2022). (6.6 IF).
• Rizwan, Hafiz Muhammad, Fang Shaozhong, Xiaoting Li, Muhammad Bilal Arshad, Ahmed Fathy Yousef, Yang Chenglong, Meng Shi et al. "Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions." Frontiers in plant science 13 (2022). (6.6 IF)
• Xiao, Yibo, Jianying Guo, Huachang Zhu, Anwar Muhammad, Haiteng Deng, Zhangli Hu, and Qingyu Wu. "Inhibition of glucose assimilation in Auxenochlorella protothecoides by light." Biotechnology for biofuels (2020). (7.6 IF).
• Guo, C., Anwar, M., Mei, R., Li, X., Zhao, D., Jiang, Y., ... & Hu, Z. (2022). Establishment and optimization of PEG-mediated protoplast transformation in the microalga Haematococcus pluvialis. Journal of Applied Phycology, 34(3), 1595-1605. (IF 3.88)
• Zeng, Z., Wang, Y., Anwar, M., Hu, Z., Wang, C., Lou, S., & Li, H. (2022). Molecular cloning and expression analysis of mytilin-like antimicrobial peptides from Asian green mussel Perna viridis. Fish & shellfish.(IF 4.62)
• Ashraf, M.F., Hou, D., Hussain, Q., Imran, M., Pei, J., Ali, M., Shehzad, A., Anwar, M., Noman, A., Waseem, M. and Lin, X., 2022. Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance. International Journal of Molecular Sciences, 23(2), p.651.(6.2IF)
• Sulin Lou | Xin Lin | Chenglong Liu | Muhammad Anwar | Hui Li | Zhangli H. Molecular cloning and functional chacterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris. Algal Research 47 (2021) (5.27 IF)
• Sun, Ting, Yuting Wang, Muhammad Anwar, Sulin Lou, Yanfeng Zeng, Hui Li, and Zhangli Hu. "Short tandem target mimics inhibit Chlamydomonas reinhardtii microRNAs." Algal Research 47 (2020): .https://doi.org/10.1016/j.algal.2020.101824 (5.27 IF)
• Altaf, Muhammad Ahsan, Rabia Shahid, Ming‐Xun Ren, Freddy Mora‐Poblete, Marino B. Arnao, Safina Naz, Muhammad Anwar et al. "Phytomelatonin: An overview of the importance and mediating functions of melatonin against environmental stresses." Physiologia Plantarum 172, no. 2 (2021): 820-846. (5.0IF)
• Hussain, A., Noman, A., Arif, M., Farooq, S., Khan, M.I., Cheng, P., Qari, S.H., Anwar, M., Hashem, M., Ashraf, M.F. and Alamri, S., 2021. A basic helix-loop-helix transcription factor CabHLH113 positively regulate pepper immunity against Ralstonia solanacearum. Microbial Pathogenesis, 156, p.104909. (3.84IF)
• Zaynab, Madiha, Yasir Sharif, Mahpara Fatima, Muhammad Zohaib Afzal, Mehtab Muhammad Aslam, Muhammad Fahad Raza, Muhammad Anwar et al. "CRISPR/Cas9 to generate plant immunity against pathogen." Microbial Pathogenesis (2020): 103996. https://doi.org/10.1016/j.micpath.2020.103996 (3.84IF).
• Adnan, Muhammad, Waqar Islam, Ali Noman, Ansar Hussain, Muhammad Anwar, Muhammad Umar Khan, Waheed Akram, Muhammad Furqan Ashraf, and Muhammad Fahad Raza. "Q-SNARE protein FgSyn8 plays important role in growth, DON production and pathogenicity of Fusarium graminearum." Microbial Pathogenesis 140 (2020): 103948. (3.84 IF).
• Irshad M, Rizwan HM, Debnath B, Anwar M, Li M, Liu S, He B, Qiu D. Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.). HortScience. 2018 Feb 1;53(2):183-90. (1.45 IF)
• Hussain, A., Noman, A., Khan, M. I., Zaynab, M., Aqeel, M., Anwar, M., ... & Bakhsh, A. (2019). Molecular regulation of pepper innate immunity and stress tolerance: An overview of WRKY TFs. Microbial pathogenesis, 103610. (3.84IF)
• Noman, A., M. Aqeel, N. Khalid, W. Islam, T. Sanaullah, M. Anwar, S. Khan, W. Ye, and Y. Lou. "Zinc finger protein transcription factors: Integrated line of action for plant antimicrobial activity." Microbial pathogenesis (2019). (3.84IF)
• Muhammad Arif , Saif Ul Islam, Muhammad Adnan, Muhammad Anwar, Habib Ali, Zujian Wu. Recent progress on gene silencing/suppression by virus-derived small interfering RNAs in rice viruses especially Rice grassy stunt virus .Microbial pathogenesis.2018.Vol.125,page210-218. (https://doi.org/10.1016/j.micpath.2018.09.021( 3.84 IF)
• Wang, G., Yang, B., Wu, J., Luo, P., Anwar, M., Allan, A. C., ... & Zeng, L. (2018). Identification of genes involved in flavonoid biosynthesis of Chinese Narcissus (Narcissus tazetta L. var. chinensis). Plant Molecular Biology Reporter, 36(5-6), 812-821. (1.8 IF)
• WU Jiacheng, WANG Guiqing, Muhammad Anwar, ZENG Lihui. 2018. Cloning and Functional Analysis of R2R3-MYB gene NtMYB5 in Narcissus tazetta var. Chinensis.ACTA HORTICULTUER SINICA, 2018, 45(7):1327-1337.
• Wang Guiqing ,Yao Hong, Jiacheng Wu,Muhammd Anwar, Zeng Lihui.Cloning and functional characterization of NtMYB7 gene in Chinese Narcissus tazetta Var. Chinensis. Acta Bot. Boreal. Occident.Sin. 2018,38(8).1401-1410.
• ALTAF, MA, R. SHAHID, MX REN, S. NAZ, MM ALTAF, A. QADIR, M. ANWAR, A. SHAKOOR, and F. HAYAT. "Exogenous melatonin enhances salt stress tolerance in tomato seedlings." BIOLOGIA PLANTARUM 64 (2020): 604-615. (1.74 IF)
• Muhammad Anwar*, Hakim Ali Sahito*1, Imran Hassan*, Nadeem Akhtar Abbasi*, Hafiz Ashfaque Ahmed*, Muhammad Ali Bhatti**, Ansar Hussain*, Zaffar Iqbal* and Azmat Hussain Abro. Effect of pre harvest treatment of salicylic on growth and vase life of tuberose with aroma environment.Wudpecker Journal of Agriculture Research.Vol.3(2),PP.050-057,February2014.
• Anwar M, Sahito HA, Butt SJ, Blouch A, Abro AH (2013).Which soilless and soil based media support the performance and quality of marigold most? Int. J. Agric.Policy Res.1 (10):298-304.
• Zaynab, M., Kanwal, S., Hussain, I., Qasim, M., Noman, A., Iqbal, U., Ali, G.M., Bahadar, K., Jamil, A., Sughra, K.,Rehman, N., Buriro, M., Abbas, S., Ali, M., Alvi, A.H., Anwar, M., Khan, M.I., Tayyab, M., 2017. Rice Chitinase Gene Expression in Genetically Engineered Potato Confers Resistance against Fusarium solani and Rhizictonia solani. PSM Microbiol., 2(3): 63-73.
• Ansar Hussain1 Muhammad Anwar*1 Adeel Anwar3 Muhammad Nadeem2 Nazam Hussain2.. To Evaluate Efficient Use of Nitrogen and Sulphuric Acid Application Towards Increase in Seed Cotton Yield and Soil Health. Journal of Biology, Agriculture and Healthcare. ISSN 2224-3208 (Paper) ISSN 2225-093X (Online). Vol.8, No.1, 2018. Corresponding author
• Raheel, M., Zaynab, M., Anwar, M., Hussain, A., Kanwal, S., Bahuder, K., Alvi, A., Ashraf, M.F., Iqbal, Z., Nadeem, M., 2017. Performance of Different Genotypes of Gossypium hirsutum under Various Sowing Conditions on Yield Contributing Parameters. PSM Biol. Res., 2(3): 133-136.
• Zaynab, M., Kanwal, S., Abbas, S., Fida, F., Islam, W., Qasim, M., Rehman, N., Fida, F., Furqan, M., Rizwan, M.,Anwar, M., Hussain, A., Tayab, M., 2017. Bioinformatics Tools in Agriculture: An Update. PSM Biol. Res., 2(3): 111-116.
• Ahmad S, Ali F, Hameed MS, Anwar M, Raqeeb A (2017) Molecular Characterization and Phylogenetic Analysis of Colletotrichum Species Associated with Anthracnose Disease. J Bacteriol Mycol Open Access 5(2): 00129. DOI: 10.15406/jbmoa.2017.05.00129.
• Ahmad S, Raqeeb A, Ali F, Hameed MS, Anwar M (2018) Characterization of Novel Antibiotic Resistance Genes in Staphylococcal aureus.JBacteriol Mycol Open Access 6(1): 00167. DOI: 10.15406/jbmoa.2017.06.00167.
• Shah, A. H., Rattar, T. M., Zhang, D., Tian, L., Solangi, Z. A., Rattar, Q. A., ... & Anwar, M. (2022). Heterosis and correlation studies in F1 hybrids of hexaploid wheat (Triticum aestivum L.) cultivars.
• Batyrbek, M., Akhtar, M., Memon, S. A., Saleem, M., Larik, R., Abdelghany, A. E., ... & Anwar, M. (2023). Influence of drought stress on Grain Yield, Yield Components of wheat cultivars under controlled condition. Journal of Biotechnology, Fieldcrops and Molecular Sciences, 1(1), 44-52.

• Hussain, Ansar, Muhammad Ifnan Khan, Muhammad Arif, Shahid Farooq, Madiha Zainab, Ali Raza, Muhammad Anwar et al. "Plant Chemical Ecology: The Focused Arena for Plant Survival and Productivity." In Developing Climate-Resilient Crops, pp. 139-158. CRC Press, 2021.
• Naz, Misbah, Muhammad Ammar Raza, Muhammad Rahil Afzal, Muhammad Jafar Jaskani, Muhammad Anwar, Muhammad Tariq, Muhammad Riaz, and Xiaorong Fan. "Microbial systems, current trends, and future prospective: a systemic analysis." In Microbial Consortium and Biotransformation for Pollution Decontamination, pp. 81-94. Elsevier, 2022.
• Fruit characterization of widely known date palm cultivars in Pakistan.

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