New Azo Amide Compounds Derived from Benzocaine Synthesis, Characterization, and Study of Biological Activity

Abstract

Benzocaine (0.05 mol, 7.42 g) was decomposed in NaNO2 in the presence of HCl at temperatures ranging from 0 to 5 degrees Celsius to produce several novel derivatives of Azo compounds (50 mL). 2-Nitrophenol (80%, 7.5 mL) and compound 1 (0.05 mol, 10.67 g) were heated for two hours at reflux in 50 mL of ethanol. When the procedure was stopped, the mixture had cooled to a normal temperature. Filtered and water was used to clean the precipitate. The ethanol yield solidified. This reaction was examined using Thin-Layer Chromatography (TLC). Melting points, elemental analyses, FTIR, 1H-NMR, and 13C-NMR were used to characterize all novel compounds. The antibacterial activity of these derivatives was also established, as was the anti-breast cancer activity of three of these compounds.

Keywords: Benzocaine; Azo Compunds; Amide Compunds; Anticancer and Anti-Microbial

Citation

Asghar A, Ali H. New Azo Amide Compounds Derived from Benzocaine Synthesis, Characterization, and Study of Biological Activity. WebLog J Amino Acids. wjaa.2026.i1804. https://doi.org/10.5281/zenodo.23104519