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ARTICLE TYPE : REVIEW ARTICLE

Published on :   11 Jun 2026, Volume - 2
Journal Title :   WebLog Journal of Cancer and Clinical Research | WebLog J Cancer Clin Res | WJCACR
Journal ISSN:   3143-1062
Source URL:   weblog icon https://weblogoa.com/articles/wjcacr.2026.f1107
Permanent Identifier (DOI) :   doi icon https://doi.org/10.5281/zenodo.20953165

Application of Genomics in CAR T-Cell Therapy: A Comprehensive Review

Jyothi Basini 1 *
O Divya Rekha 2
Hemalatha Jalli 3
1Professor and Head, Department of Pharmacology, Seven Hills College of Pharmacy (Autonomous), Venkatramapuram, Tirupati-517561, AP, India
2Associate Professor, Department of Pharmacy Practice, Seven Hills College of Pharmacy (Autonomous), Venkatramapuram, Tirupati-517561, AP, India
3Pharm.D Intern, Department of Pharmacy Practice, Seven Hills College of Pharmacy (Autonomous), Venkatramapuram, Tirupati-517561, AP, India

Abstract

Cancer is a genetically driven disease characterized by the progressive accumulation of somatic mutations, chromosomal aberrations, epigenetic dysregulation, and transcriptional reprogramming that collectively promote malignant transformation and immune escape. This genomic instability generates profound tumour heterogeneity, driving therapeutic resistance, immune evasion, and disease relapse. These challenges have accelerated the development of precision immunotherapies, such as chimeric antigen receptor (CAR) T-cell therapy, that leverage genetic engineering to overcome tumour complexity. CAR T cell therapy has emerged as a transformative approach for treating haematological malignancies. Cancer which is one of the comprehensive burdens responsible for approximately 20 million new cases and 10 million deaths globally each year, reqiures innovative treatments such as CAR T cell therapy are increasingly essential for managing refractory and difficult-to-treat cancers. The integration of genomics into CAR T cell research and clinical practice has substantially advanced target discovery, patient stratification, therapy optimization, and safety evaluation. Cutting-edge technologies including next-generation sequencing, single cell genomics, transcriptomics, and Clustered Regularly Interspaced Short Palindromic Repeats screening have provided crucial insights into tumour heterogeneity, antigen expression, immune evasion, and CAR T cell functionality. This review summarizes the classification and mechanisms of CAR T cell therapies and highlights the diverse applications of genomics in their development and clinical implementation, alongside current limitations and future perspectives.

Keywords: CAR T cell Therapy; Genomics; Clustered Regularly Interspaced Short Palindromic Repeats; Next Generation Sequencing; Transcriptomics; Immunotherapy; Tumour Heterogeneity; Antigen Expression

Citation

Basini J, Divya Rekha O, Jalli H. Application of Genomics in CAR T-Cell Therapy: A Comprehensive Review. WebLog J Cancer Clin Res. wjcacr.2026.f1107. https://doi.org/10.5281/zenodo.20953165