Name: Ghada Mohamed Shehata Nour Eldeen

Work Address: National Research Centre, El-Behooth St.,Dokki, Cairo, Egypt,

Human Genetics and Genome Research Division, Department of Molecular Genetic Enzymology.

E-mail Address: genoghada@ymail.com

Phone No: 01007196882

Last Scientific Academic Degree Qualification:

-MD. Degree in clinical and chemical pathology Department- Immunology subdivision, Faculty of medicine, Cairo University. Excellent with honor. October / 2001

Last Professional Career:

2021-till now: associate professor. Department of Molecular Genetic Enzymology, National Research Centre.

Skills and Practical Experience

• Adult stem cells propagation in the laboratory, handling and preservation under aseptic settings starting since 2013.
• Optimizing culture systems, extracellular matrix components, and exogenous growth factors for the enrichment and expansion of adult stem cells.
• Isolation of primary cells: hMSCs (form bone marrow, umbilical cord blood, Wharton`s jelly, adipose tissue), human mononuclear cells from peripheral blood.
• Development of an efficient system for culture and maintenance of human mesenchymal stem cells.
• Experience in a range of tissue culture techniques including 3D and Co-culture systems.
• Experience in stable cell line generation
• Different differentiation techniques to different cell types (osteogenic, chondrogenic linage, and insulin secreting cells).
• Imaging, light, inverted, and fluorescence microscopy
• Experience in molecular biology techniques (DNA/RNA/protein) since 2004
• Experience required in cellular and biochemical assay development and execution: cell culture and ELISA; flow cytometry, and imaging techniques.

Scientific Projects

• Dynamic micro-carrier scale-up toward human mesenchymal stem cell- based medicinal product(NRC), 2023- (principal investigator).
• Epigenetic targeted therapy of inflammasome -related disorders towards precision medicine of autoinflammatory diseases (NRC), 2023- (member).
• Establishment of an efficient and cost effective quality control system for cell-based therapy products (NRC), 2023- (member).
• New drug-like small molecule antagonizes phosphatidylinositol-3,4,5-triphosphate(PIP3) binding to pleckstrin homology domain of protein kinase B/ Akt, extended in vitro mesenchymal stem cell-derived cardiomyocytes culture evaluation study(NRC), 2023- (member).
• Investigating the impact of diabetes mellitus on human adipose derived mesenchymal stem cells immunomodulatory based therapy and evaluating interferon-gamma priming potential to improve this therapeutic approach. (STDF), 2021-2023, (member)
• Assessment of diabetes mellitus type 2 impact on human adipose derived stem cell immunomodulatory based therapy and evaluating interferon-gamma priming potential to improve this therapeutic approach. (STDF), 2019-2022, (member)
• Adipose tissue -derived mesenchymal stem cells (AT-MSCs) paracrine effect and biomaterial- based tissue engineering as an innovated tool in osteoarthritis treatment (NRC), 2017-2020 (principal investigator)
• Investigating the impact of diabetes mellitus type 2 on human adipose derived mesenchymal stem cells immunomodulatory based therapy.(NRC), 2017-2020 (member)
• Bone mass among Overweight and Obese Women: Mechanism and Intervention. (NRC), 2017-2020 (member)
• Bone Repair and Regenerative Medicine applying Human derived Mesenchymal Stem Cells in combination with Scaffolds(NRC), 2014-2017 (member)
• Advanced clinical, molecular and cytogenetic studies in congenital heart disease (NRC), 2014-2017 (member)

•             Identification of Genetic Defects in Congenital Heart Disease (NRC), 2007-2010 (member)

• Genetics
• Molecular Genetics
• Immunology
• Pathology
• Enzymology

• Effect of Bio MTA plus &ProRoot MTA pulp capping materials on the regenerative properties of human dental pulp stem cells. Khaled MohamedAyoub, Mohamed Mokhtar Nagy, Riham MohamedAly, Ghada Nour El Deen& Karim El-Batouty.Scientific Reports | (2025) 15:4749 | https://doi.org/10.1038/s41598-025-88816-5
• Acquisition of durable insulin-producing cells from human adipose tissue-derived mesenchymal stem cells as a foundation for cell- based therapy of diabetes mellitus. Ghada Nour Eldeen , Hadeer A Aglan , Nadia S Mahmoud , Mazen Abdel Rasheed , Osama M Azmy , Hanaa H Ahmed. Sci Rep. 2024 Oct 18;14(1):24417.doi: 10.1038/s41598-024-74527-w.
• Enhancement of the chondrogenic differentiation capacity of human dental pulp stem cells via chondroitin sulfate-coated polycaprolactone-MWCNT nanofibers.Eldeen, G.N., Elkhooly, T.A., El Bassyouni, G.T., Hawash, A.R., Aly, R.M.ScientificReportsThis link is disabled., 2024, 14(1), 16396
• Infrared diode laser enhances human periodontal ligament stem cells behaviour on titanium dental implants. Abo El-Dahab, M.M., El Deen, G.N.,Aly, R.M., Gheith, M.Scientific Reports.This link is disabled., 2024, 14(1), 4155
• Altered expression of miR-17 and miR-148b in pediatric familial Mediterranean fever patients.Mona F Sokkar , Ghada Nour Eldeen , Randa S Lotfy , Maha M Kobesiy , Hala T El-Bassyouni , Waheba A Zarouk . Clin Rheumatology. 2024 Aug;43(8):2661-2667.
• The relation of MEFV gene variants to clinical phenotype and selected laboratory markers in Egyptian patients with familial Mediterranean fever. Journal of The Arab Society for Medical Research. El-Bassyouni, HalaT.a; Eldeen, GhadaNourb; Sokkar, Mona F.b; Taher, Mohamed B.a; Sayed-Ahmed, Mohammed M.a; Salama, Iman I.c; Raslan, Hala M.d. 19(2):p 186-193, July-December 2024. | DOI: 10.4103/jasmr.jasmr_26_24
• The efficacy of infrared diode laser in enhancing the regenerative potential of human periodontal ligament stem cells (hPDLSCs). Mohamed M. Abo El-Dahab, Ghada Nour El Deen, Mahmoud Shalash, Mostafa Gheith, Ahmed Abbass& Riham M. Aly. BMC Oral Health volume 24, Article number: 1310 (2024)
• The regenerative potential of Tideglusib and CHIR99021 small molecules as potent odontogenic differentiation enhancers of human dental pulp stem cells. Hanna, S., Eldeen, G.N., Alfayate, R.P., Aly, R.Clinical Oral Investigations. This link is disabled., 2024, 28(1), 48
• Small Molecule GSK-3 Inhibitors Safely Promote the Proliferation and Viability of Human Dental Pulp Stem Cells-In Vitro (2023).Samer Hanna, Riham Aly, Ghada Nour Eldeen, Ruth Perez Alfayate,.Biomedicines 11(2):542DOI: 10.3390/biomedicines11020542
• Optimization of differentiation protocols of dental tissues stem cells to pancreatic β-cells Riham M. Aly ,Hadeer A. Aglan , Ghada Nour Eldeen and Hanaa H. Ahmed.l. BMC Molecular and Cell Biology (2022) 23:41
• Efficient generation of functional pancreatic β cells from dental-derived stem cells via laminin-induced differentiation (2022). Riham M. Aly, Hadeer A. Aglan, Ghada Nour Eldeen , Nadia S. Mahmoud, Eman H. Aboul Ezz, and Hanaa H. Ahmed. Journal of Genetic Engineering and Biotechnology 20:85
• Dynamic disequilibrium-based pathogenicity model in mutated pyrin’s B30.2 domain—Casp1/p20 complex Alaaeldin G. Fayez ,Ghada Nour Eldeen , Waheba A. Zarouk , Khaled Hamed , Abeer Ramadan , Bardees M. Foda, Maha M. Kobesiy , Mai E. Zekrie , Randa S. Lotfy , Mona F. Sokkar and Hala T. El Bassyouni.. Journal of Genetic Engineering and Biotechnology. (2022) 20:31.
• Impact of type 2 diabetes mellitus on the immunoregulatory characteristics of adipose tissue-derived mesenchymal stem cells(2021).Nourhan Abu-Shahba, Marwa Mahmoud,Alaa Mohammed ElErian,Mohamed Ibrahim Husseiny,Ghada Nour Eldeen,Iman Helwa,Khalda Amr,Mahmoud ElHefnawi,Amel Ibrahim Othman ,Sherif Abdelaziz Ibrahim,OsamaAzmy.The International Journal of Biochemistry & Cell Biology, Vol. 140, November 2021, 106072
• Narrative role of vitamin D receptor with osteoporosis and obesity in a sample of Egyptian females: a pilot study.Hassan NE, El-Masry SA, Zarouk WA, Eldeen GN, Mosaad RM, Afify MAS, Aly MM, Khalil A. J Genet EngBiotechnol. 2021 Aug 5;19(1):115. doi: 10.1186/s43141-021-00216-0. PMID: 34351532; PMCID: PMC8339694.
• Clinical Implications of S100A12 and Resolvin D1 Serum Levels, and Related Genes in Children with Familial Mediterranean Fever. Zeinab Y. Abdallah , Mona Ibrahim , Manal M. Thomas  , Hisham Megahed , Ghada Nour Eldeen , Khaled Hamed , Mohamed Fares , Mahmoud ElHefnawi , Hala T. El-Bassyouni. CC BY 4.0 • Journal of Child Science 2021; 11(01): e163-e169. DOI: 10.1055/s-0041-1731303
• Association of some dietary ingredients, vitamin D, estrogen, and obesity polymorphic receptor genes with bone mineral density in a sample of obese Egyptian women. Hassan NE, El Shebini SM, El-Masry SA, Ahmed NH, Eldeen GN, Rasheed EA, Aly MM, Alian KM, Afify MAS, Khalil A.J Genet EngBiotechnol. 2021 Feb 9;19(1):28. doi: 10.1186/s43141-021-00127-0. PMID: 33559788; PMCID: PMC7873164.
• Adipose tissue-derived mesenchymal stem cells and chitosan / poly (vinyl alcohol) nanofibrous scaffolds for cartilage tissue engineering (2020). Ghada Nour Eldeen, Mazen Abdel-Rasheed, Amira M. El-Rafei, Osama Azmy, Gehan T. El-Bassouni. Cell Regeneration 9:7
• Carotid intima- media thickness, lipid profile, serum amyloid A and vitamin D status in children with familial Mediterranean fever.Ramy Mohamed, Hala T. El-Bassyouni, SherifHasanElwan, EmanYouness, Doaa R. Soliman, Ghada M. Shehata, MoushiraErfanZaki. The Egyptian Rheumatologist 42(2020)237-240.
• Multiple cytokine analysis in familial Mediterranean fever Egyptian cases using multiplex assay (Luminex technology) as a new inflammatory biomarker for disease activity. Naglaa Kholoussi , Shams Kholoussi , Hala T. El Bassyouni , Haiam A. Raouf , Iman Helwa , Mohamed I. Gadelhak , Ghada N. Eldeen , HalaElnady. Middle East Journal of Medical Genetics: July 11, 2020, IP: 10.232.74.26
• Optimizing a serum-free/xeno-free culture medium for culturing and promoting the proliferation of human dental pulp stem cells (2019). Esraa Mohamed Abdel Moniem, Mona Mahmoud EL-Batran, Ahmed Mahmoud Halawa, Dina Hazem Gomaa, Ghada Nour Eldeen, Riham Mohamed Aly. Stem Cell Investigation; 6: (15) 1–10.
• Apoptosis, reactive oxygen species and DNA damage in Familial Mediterranean Fever patients.Wagdy Khalil Bassaly Khalil, WahebaZarouk, Ghada Nour Eldeen,Abeer Ramadan, Alaaeldin Fayez, Nora Esmaiel, BardeesFoda, Khaled Hamed, Salwa Mohamed Kassem, Hala EL-Bassyouni. Gene Reports (2019)76-80.
• Non-target Genes Regulate miRNA-Mediated Migration of Steering of Colorectal Carcinoma. Sohair M. Salem, Ahmed R. Hamed, AlaaeldinG.Fayez, Ghada Nour Eldeen. Pathology and Oncology Research (2018).
• Nano Hydroxyapatite &Mineral Trioxide Aggregate Efficiently Promote Odontogenic Differentiation of Dental Pulp Stem Cells. Ahmed Khaled Hanafy,SouzyF.Shinaishin,Ghada Nour Eldeen,Riham Mohamed Aly. Macedonian Journal of Medical Science.2018,Sep.25;6(9):1727-17
• Has the time come to include low-laser photobiomodulation as an adjuvant therapy in the treatment of impaired endometrial receptivity?. DoaaAbdelmageedElFaham, Mohamed Amr Hussein Elnoury, Mona IbraheimMorsy, MarwaAbdelmoneimElShaer, Ghada Mohammed Nour Eldin, Osama Mahmoud Azmy. Lasers in Medical Science (2018) 33:1105-1114.
• Differentially expressed genes: OCT-4, SOX-2, STAT3, CDH1 and CDH2, in cultured mesenchymal stem cells challenged with serum of women with endimetriosis. Journal of Genetic Engineering and Biotechnology. Ehab Salama, Ghada Nour Eldeen, Mazen Abdel-Rasheed, Sahar Abdel Atti, Tamer Taha, Osama Azmy. 16 (2018) 63-69
• Assessment of physical growth, some oxidative stress biomarkers and vitamin D status in children with Familial Mediterranean Fever. Moushira Zaki, Hala T. El-Bassyouni, Hanaa Reyad, Walaa Yousef, EmanYouness, Ghada Mohamed, Abeer Ramadan. Meta Gene (2018)212-215.
• Screening of the most common MEFV mutations in a large cohort of Egyptian patients with Familial Mediterranean Fever. Waheba A. Zarouk, Hala T. El-Bassyouni, Abeer Ramadan, Alaaeldin G. Fayez ,Nora N. Esmaiel , Bardees M. Foda, Maha M. Kobiesy, May E. Zekry, Randa S. Lotfy, Ghada M. Shehata. ISSN24520144. Vol.11,23-28. Gene Reports, 2018
• microRNA expression analysis in endometriotic serum treated mesenchymal stem cells. Mazen Abdel-Rasheed,Ghada Nour Eldeen, Marwa Mahmoud, Mahmoud ElHefnawi, Nourhan Abu-shahba, Mohamed Reda, Khaled Elsetohy, Michael Nabil, Amr Elnoury, Tamer Taha, Osama Azmy. EXCLI Journal. (2017) ;16:852-867-ISSN 1611-2156.
• Prediction of the functional consequences of a novel homozygous TBX5 variant in isolated AVSD patient. Alaaeldin. G. Fayez, Nora N. Esmaiel ,Arwa A. El Darsh , Engy A. Ashaat , Alaa K. Kamel , Ghada M. Shehata , Azza M. Ahmed , Mona O. El RubyJournal of Innovations in Pharmaceutical and Biological Sciences (JIPBS) ISSN: 2349-2759. Vol 4 (3), 97-105, 2017

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